Terminator

Part:BBa_K780000

Designed by: Nikodem Latocha   Group: iGEM12_Warsaw   (2012-09-26)

Terminator.png

Terminator for Bacillus subtilis

  • Our terminator is based on strong, Rho-independent terminator of gyrase gene, which is type II topoisomerase (EC 5.99.1.3). DNA gyrase uses the energy of ATP hydrolysis to introduce negative supercoils or relaxes positive supercoils.
  • Negative DNA supercoiling is essential in vivo to compact the genome, relieve torsional strain during replication, and promote local melting for vital processes such as transcript initation by RNA polymerase.
  • Gyrase gene is a housekeeping gene and because of that it must have efficient terminator.

Secondary Structure

Mfold-K780000-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
[edit]
Categories
//chassis/prokaryote/bsubtilis
//direction/forward
//terminator/single
Parameters
None